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Glycogen phosphorylase is a key enzyme in glycogen catabolism, catalyzing glycogen phosphorylation. Glycogen phosphorylase A (GPa) and Glycogen phosphorylase B (GPb) can be divided into two forms. The decomposition of glycogen is mainly catalyzed by glycogen phosphorylase A. When no activator was added, glycogen phosphorylase a catalyzed glycogen and inorganic phosphorus to produce glucose residue to generate glycogen and glucose 1-phosphate, and glucose phosphate mutase and 6-phosphate glucose dehydrogenase further catalyzed NADP to reduce NADPH in sequence. The increasing rate of NADPH could reflect the activity of glycogen phosphorylase a at 340nm. The total GP (GPa and GPb) activity was measured with the addition of activator, and the GPb activity was obtained by gP-GPA activity without the addition of activator.
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