Creatinine (Cr), C4H7N3O, is a product of muscle metabolism in the human body, mainly through the glomerular filtration out of the body. Creatinine in blood comes from exogenous and endogenous two kinds, exogenous creatinine is the product of meat metabolism in the body; Endogenous creatinine is the product of metabolism of muscle tissue in the body. Creatine is hydrolyzed to creatine by creatinase. Under the catalysis of creatine enzyme hydrolysis creatine and urea. Sarcosine is oxidized by sarcosine oxidase to produce hydrogen peroxide. Peroxidase catalyzes the oxidation of 4-amino-antipyrine conjugated phenols by hydrogen peroxide to produce colored compounds with characteristic absorption peaks at 505nm.

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