Product Instruction：

Nucleotide has important biological functions. It is a kind of compound composed of purine base or pyrimidine base, ribose or deoxyribose and phosphoric acid, and mainly participates in the formation of nucleosides.

Adenosine triphosphate (ATP) is considered a universal energy source essential for cellular synthesis in the survival and reproduction of all living organisms. ATP is produced by a variety of cellular pathways. The most typical is phosphorylation by peroxidation in the mitochondria, which is synthesized by atp synthase, or by photosynthesis in the chloroplasts of plants. The main energy sources for ATP synthesis are glucose and fatty acids.

Adenosine diphosphate (ADP) is widely present in animals, plants, microorganisms and cultured cells. In organisms, the loss of a phosphate radical is usually the result of hydrolysis of adenosine triphosphate (ADP), that is, breaking a high-energy phosphate bond and releasing energy.

Adenosine monophosphate (AMP) is widely found in animals, plants, microorganisms and cultured cells, and is formed after the release of energy by ATP and AMP in the body. It can continue to bind phosphate groups to form adenosine diphosphate (AMP) and adenosine triphosphate (ATP). It's an incomplete hydrolysis of ATP.

ATP, ADP and AMP have absorption peaks at 254 nm, and HPLC can be used to determine different nucleotide contents according to different peak time and peak area.

Instruments and reagents required in the test:

High Performance Liquid chromatograph (C18 column (4.6×250 mm), UV detector (VWD), bench centrifuge, adjustable pipette, mortar/homogenizer, Brown EPT, needle filter (50, water system, 0.45 µm), Syringe, extractor, filter film (organic system, water system), brown into S flask (50, 2 mL), acetonitrile (chromatographic pure, 500 mL), ultrapure water.

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