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BCA Protein Assay Kit
BCA Protein Assay Kit
大黄素甲醚含量检测试剂盒
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BCA Protein Assay Kit

Cat: AK0651-50T

Size: 50T

Storage: BSA protein standard keeps at 4for 3 monthsor at -20for 1 year, other reagents keep at room temperature for 1 year.

Components:

 

50T

 

Storage

BCA reagent

100ml

 

RT

Cu reagent

3ml

 

RT

PBS dilution

30ml

 

RT

BSA Standard(5mg/ml)

1ml

 

-20

Product Description:

The BCA Protein Assay Kit is based on the protein reduces Cu2+ to Cu+ and the principle that BCA forms a purple-blue complex with Cu1+ under alkaline condition, followed by detecting absorbance at 562nm and comparing with standard curve to quantify the protein. Commonly used detergents SDS, Triton X-100, Tween do not affect the test results, but The BCA Protein Assay Kit is affected by chelating agents (EDTA, EGTA), reducing agents (DTT, mercaptoethanol) and lipids. During the experiments, when the high background caused by sample dilution or lysate, it is advisable to take  the Bradford Protein Assay Kit into account.

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