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Product Description: Caspase is a family of proteases involved in the process of apoptosis, including more than 10 members. Caspase-6, also known as Mch-2, its precursor protein is cleaved by granzyme B to form activated caspase-6 dimer, which can induce apoptosis. Caspase- 6 can cleave PARP, a key regulator of apoptosis, and Lamin A, a key protein on the nuclear membrane. Its recognition site for Lamin A is VEID. The caspase-6 colorimetric assay is based on the hydrolysis of the peptide substrate AcVETD-pNA (N-acetyl-Val-Glu-Ile-Asp-p-nitroanilide) by caspase-6, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell.
Reagents and Equipment Required but Not Provided: Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water
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