Determination significance: Urea is the final product of decomposition of nitrogen compounds in organisms, which is converted into ammonia under the catalysis of urinary enzyme. Blood urea nitrogen is one of the main indexes of renal function.

Determination principle: under alkaline condition, urine enzyme hydrolyzes urea to produce ammonia, which reacts with hypochlorous acid to produce chloramine, and then reacts with phenol derivative to produce green indoxol, which has characteristic absorption peak at 630nm.

Required instruments and consumables: balance, mortar, normal temperature centrifuge, visible spectrophotometer/microplate reader, quartz cuvette / 96-well plate, constant temperature water bath.

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