Hepatic lipase (HL) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer

Cat No: AK0233

Size:50T/24S

Components:

Reagent I: 100 mL×1. Storage at 4℃ .

Reagent II: 3 mL×1. Storage at 4℃ .

Reagent III: Powder×1. Storage at 4℃ . Before use, add 30 mL of distilled water, fully dissolve.

Reagent IV: Powder×2. Storage at -20℃ . Before use, add 2 mL of distilled water to the one, fully dissolve. The dissolved reagent can be stored at -20°C after repacking. Avoid repeated freeze-thaw cycles;

Standard: Powder×1. Before use,  6.94 mL of acetone is added to prepare a  10  μmol/mL  α-naphthol standard solution, which was fully dissolved before use.

Product Description:

Hepatic lipase (HL) is a lipolytic enzyme synthesized in liver parenchymal cells. It is present on the surface  of the  liver  sinusoidal  endothelial  cells  and  the  surface  of the  hepatocyte  microvilli  in  the sinusoidal space, and can hydrolyze various lipoproteins. The triglycerides (TG) and phospholipids (PL) in the medium change the size and density of various lipoprotein particles. When the HL and its activity in the plasma increasing, it can lead to low density lipoprotein (LDL) levels in the plasma, increase and accelerate the occurrence and development of atherosclerosis.

HL hydrolyzes α-naphthyl acetate to produce α-naphthol, which can form a purple-red azo compound with fast blue B  salt.  It has  a  characteristic  absorption peak  at  595 nm,  and  its  color  depth  is positively correlated with liver esterase activity within a certain range.

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