Drug Names

Generic Name：Mouse Mycoplasma ELISA Kit

Purpose

This kit allows for the determination of Mycoplasma concentrations in Mouse serum, blood plasma, and other biological fluids.

Principle

The kit assay Mouse Mycoplasma level in the sample，use Purified Mouse Mycoplasma antibody to coat microtiter plate wells, make solid-phase antibody, then add Mycoplasma to wells, Combined With Mycoplasma, after washing and removing non-combinative antigen and other components ,then Combined Mycoplasma antibody which with HRP labeled become antibody - antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge Mycoplasma exist in the sample or not.

 

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