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Purpose
Our Mouse Xanthine Oxidase(XOD)ELISA kit is to assay Xanthine Oxidase(XOD) levels in Mouse serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Xanthine Oxidase(XOD). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Xanthine Oxidase(XOD) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Xanthine Oxidase(XOD)and HRP conjugated Xanthine Oxidase(XOD) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Xanthine Oxidase(XOD). You can calculate the concentration of Xanthine Oxidase(XOD) in the samples by comparing the OD of the samples to the standard curve.
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