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Drug Names
Generic Name: Mouse Mature Brain-Derived Neurotrophic Factor (mBDNF) ELISA Kit
Purpose
Our Mouse Mature Brain-Derived Neurotrophic Factor (mBDNF) ELISA kit is to assay mBDNF levels in Mouse serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to mBDNF. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for mBDNF is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain mBDNF and HRP conjugated mBDNF antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of mBDNF. You can calculate the concentration of mBDNF in the samples by comparing the OD of the samples to the standard curve.
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