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Rat inhibin b ,INH-B ELISA Kit
Rat inhibin b ,INH-B ELISA Kit
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  • NO.:SL0366Ra
    Assay range:3.3pg/ml -200 pg/ml
    Sensitivity:0.6pg/ml
    Standard:270 pg/ml
    Sample Volume:10ul
    Wavelength:450nm
  • Goods click count:111
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Purpose

Our Rat Inhibin B (INHB) ELISA kit is to assay INHB levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to INHB Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for INHB is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain INHB and HRP conjugated INHB antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of INHB. You can calculate the concentration of INHB in the samples by comparing the OD of the samples to the standard curve.

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