Drug Names

Generic Name：Goat  Orf virus disease antibody IgG(ORFV-IgG) ELISA Kit

Purpose

Our Goat Orf virus disease antibody IgG(ORFV-IgG) ELISA kit is to assay ORFV-IgG levels in Goat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to ORFV-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for ORFV is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ORFV-IgG and HRP conjugated ORFVantigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of ORFV-IgG is determined by comparing with the CUTOFF value.

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