Drug Names

Generic Name：Rat Complement Component 5 (C5) ELISA Kit

Purpose

Our Rat Complement Component 5 (C5) ELISA kit is to assay C5 levels in Rat’s serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to C5. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for C5 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain C5 and HRP conjugated C5 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of C5. You can calculate the concentration of C5 in the samples by comparing the OD of the samples to the standard curve.

 

 

 

 

 

 

Materials provided with the kit

	Materials provided with the kit	96 determinations	Storage
1	User manual	1	R.T.
2	Closure plate membrane	2	R.T.
3	Sealed bags	1	R.T.
4	Microelisa stripplate	1	2-8℃
5	Standard：5400pg/ml	0.5ml×1 bottle	2-8℃
6	Standard diluent	1.5ml×1 bottle	2-8℃
7	HRP-Conjugate reagent	6ml×1 bottle	2-8℃
8	Sample diluent	6ml×1 bottle	2-8℃
9	Chromogen Solution A	6ml×1 bottle	2-8℃
10	Chromogen Solution B	6ml×1 bottle	2-8℃
11	Stop Solution	6ml×1 bottle	2-8℃
12	wash  solution	20ml (30X)×1bottle	2-8℃

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