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Purpose
Our Rat Peroxiredoxin 2 (PRDX2) ELISA kit is to assay Peroxiredoxin 2 (PRDX2)levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 2 (PRDX2) . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Peroxiredoxin 2 (PRDX2) added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Peroxiredoxin 2 (PRDX2) and HRP conjugated Peroxiredoxin 2 (PRDX2) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Peroxiredoxin 2 (PRDX2) . You can calculate the concentration of Peroxiredoxin 2 (PRDX2) in the samples by comparing the OD of the samples to the standard curve.
Username | Quantity | bought time |
Qu*** | 1 | 2024-04-30 |
Fe*** | 1 | 2021-12-13 |
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