Drug Names

Generic Name：Rat Acetyl-CoA acetyltransferase,mitochondrial(ACAT1)ELISA Kit

Purpose

Our Rat Acetyl-CoA acetyltransferase,mitochondrial(ACAT1)ELISA kit is to assay ACAT1

levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided

in this kit has been pre-coated with an antibody specific to ACAT1. Standards or samples are

added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for ACAT1 is added to

each Microelisa stripplate well and incubated. Free components are washed away. The TMB

substrate solution is added to each well. Only those wells that contain ACAT1 and HRP

conjugated ACAT1 antibody will appear blue in color and then turn yellow after the addition

of the stop solution. The optical density (OD) is measured spectrophotometrically at a

wavelength of 450 nm. The OD value is proportional to the concentration of ACAT1. You can

calculate the concentration of ACAT1 in the samples by comparing the OD of the samples to

the standard curve.

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