Drug Names

Generic Name：Rat Programmed Cell Death Protein 1 (PD-1)ELISA Kit

Purpose

Our Rat  Programmed Cell Death Protein 1 (PD-1) ELISA kit is to assay  Programmed Cell Death Protein 1 (PD-1)  levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to  Programmed Cell Death Protein 1 (PD-1) . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for  Programmed Cell Death Protein 1 (PD-1) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Programmed Cell Death Protein 1 (PD-1)  and HRP conjugated  Programmed Cell Death Protein 1 (PD-1) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of  Programmed Cell Death Protein 1 (PD-1) . You can calculate the concentration of Programmed Cell Death Protein 1 (PD-1)  in the samples by comparing the OD of the samples to the standard curve.

 

 

 

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