TEL: +86 571 56623320 EMAIL: [email protected]
Drug Names
Generic Name:Rat Macrophage Inflammatory Protein 1 Beta (MIP-1b) ELISA Kit
Purpose
Our Rat Macrophage Inflammatory Protein 1 Beta (MIP-1b) ELISA kit is to assay MIP-1b levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to MIP-1b. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for MIP-1b is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain MIP-1b and HRP conjugated MIP-1b antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of MIP-1b. You can calculate the concentration of MIP-1b in the samples by comparing the OD of the samples to the standard curve.
Scan Wechat Qrcode