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Drug Names
Generic Name:Bovine Pasteurella multocida Antigen B2(PTM-B2)ELISA Kit
Purpose
Our Bovine Pasteurella multocida Antigen B2(PTM-B2)ELISA kit is to assayPTM-B2levels in Bovine serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PTM-B2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for PTM-B2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PTM-B2 and HRP conjugated PTM-B2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of PTM-B2 is determined by comparing with the CUTOFF value.
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