Drug Names

Generic Name：Bovine Salmonella ELISA Kit

Purpose

Our Bovine Salmonella ELISA kit is to assay Salmonella levels in Bovine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Salmonella. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Salmonella is added  to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Salmonella and HRP conjugated Salmonella antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at  a wavelength of 450 nm. The presence of Salmonella is determined by comparing with the CUTOFF value.

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