Drug Names

Generic Name：Bovine Mycobacterium Tuberculosis antibody (TB-Ab) ELISA Kit

 

Purpose

Our Bovine Mycobacterium Tuberculosis antibody (TB-Ab) ELISA kit is to assay TB-Ab levels in Bovine serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to TB-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for TB is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TB-Ab and HRP conjugated TB antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of TB-Ab is determined by comparing with the CUTOFF value.

 

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