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Fish 17-alpha- methyl Testosterone (17-αMT)  ELISA Kit

Fish 17-alpha- methyl Testosterone (17-αMT) ELISA Kit

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  • Cat NO.:SL0078FI
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:3 pg/ml -120 pg/ml
  • Sensitivity:0.5 pg/ml
  • Qualitative:
  • Standard:135 pg/ml
  • Price:$528
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameFish 17-alpha- methyl Testosterone (17-αMT) ELISA Kit

 

Purpose

Our Fish 17-alpha- methyl Testosterone (17-αMT) ELISA kit is to assay 17-αMT levels in Fish serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 17-αMT. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 17-αMT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 17-αMT and HRP conjugated 17-αMT antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 17-αMT. You can calculate the concentration of 17-αMT in the samples by comparing the OD of the samples to the standard curve.

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