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Human Vitamin B(VB) ELISA Kit

Human Vitamin B(VB) ELISA Kit

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  • Cat NO.:SL3269Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:10 nmol/L - 350 nmol/L
  • Sensitivity:1 nmol/L
  • Standard:360 nmol/L
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameHuman Vitamin B(VB) ELISA Kit

Purpose

Our Human Vitamin B(VB) ELISA kit is to assay VB levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to VB. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for VB is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain VB and HRP conjugated VB antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of VB. You can calculate the concentration of VB in the samples by comparing the OD of the samples to the standard curve.

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