TEL: +86 571 56623320    EMAIL: [email protected]

General ARG/ Arginine ELISA Kit
General ARG/ Arginine ELISA Kit
Total
(Vip priceV)
Regular members: $489.6
View History [Clear]

Details

Intended use
This immunoassay kit allows for the in vitro quantitative determination of General ARG concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General ARG, During the reaction, General ARG in the sample or standard competes with a fixed amount of biotin-labeled General ARG for sites on a pre-coated Monoclonal antibody specific to General ARG. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General ARG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Arginine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-109%

107-115%

99-108%

107-116%

EDTA plasma(n=5)

97-105%

90-100%

118-127%

84-96%

heparin plasma(n=5)

88-98%

 

109-119%

105-117%

107-117%

 

 Sequence:C6H14N4O2

 

 

Partial purchase records(bought amounts latest10)

UsernameQuantitybought time
Qu***22023-12-03
Na***32022-10-04
Ta***12021-06-13
Ja***12021-04-23
Zo***22020-09-11
Total 10 records, divided into2 pages First Prev Next Last

User Comment(Total0User Comment Num)

  • No comment
Total 0 records, divided into1 pages First Prev Next Last
Username: Anonymous user
E-mail:
Rank:
Content:
Verification code: captcha

Call us

+86 571 56623320

Address

Room 1-315, Kongle Changqing Building, No. 160 Guangye Road,Gongshu District, Hangzhou City, Zhejiang Province, China

Join Us with

Leave a message
* To protect against spam, please pass the CAPTCHA test below.