Intended use
This immunoassay kit allows for the in vitro quantitative determination of General VD3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General VD3, During the reaction, General VD3 in the sample or standard competes with a fixed amount of biotin-labeled General VD3 for sites on a pre-coated Monoclonal antibody specific to General VD3. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General VD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin D3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-109%	90-99%	93-103%	108-119%
EDTA plasma(n=5)	106-114%	86-97%	109-119%	92-102%
heparin plasma(n=5)	106-116%	102-111%	99-108%	105-117%

 

Sequence:C27H44O

 

 

 

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