Intended use
This immunoassay kit allows for the in vitro quantitative determination of General ET concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General ET, During the reaction, General ET in the sample or standard competes with a fixed amount of biotin-labeled General ET for sites on a pre-coated Monoclonal antibody specific to General ET. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General ET in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endotoxin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	105-114%	95-105%	92-101%	107-117%
EDTA plasma(n=5)	89-101%	80-80%	106-118%	108-118%
heparin plasma(n=5)	111-120%	95-104%	101-113%	104-113%

 

General Annotation

Function:

Endotoxins are a component of the cell wall of certain bacteria. Bacteria which contain endotoxin are known as gram negative bacteria. The endotoxins are released from the bacteria as they are destroyed and elicit a strong response from the immune system by activating various components of the immune system and inducing cytokine release.

 

 

 

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