Drug Names

Generic Name：Cucumber Mosaic Virus(CMV) ELISA Kit

Purpose

Our Cucumber Mosaic Virus(CMV) ELISA kit is for the qualitative determination of CMV levels in plant tissues or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CMV Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for CMV is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CMV and HRP conjugated CMVantibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of CMV is determined by comparing with the CUTOFF value.

 

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