Drug Names

Generic Name：Plant Gibberellic Acid (GA) ELISA Kit

Purpose

Our Plant Gibberellic Acid (GA)ELISA kit is to assay Gibberellic Acid (GA) levels in plant tissues.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to  Gibberellic Acid (GA). Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for  Gibberellic Acid (GA) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Gibberellic Acid (GA) and HRP conjugated  Gibberellic Acid (GA) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of  Gibberellic Acid (GA). You can calculate the concentration of  Gibberellic Acid (GA) in the samples by comparing the OD of the samples to the standard curve.

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