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1. Purpose
This kit is tylosin Quantitative detection of residues in feed, fish, shrimp, tissue, egg, honey, milk, serum and urine sample.
2. Principle
This kit uses a direct competitive ELISA method in microplate coated with tylosin coupled antigen, adding tylosin standard or samples, free tylosin and pore strips Pre-coated tylosin conjugated antigen compete against tylosin antibody enzyme marker color with TMB substrate, stop solution added after the color from blue to yellow, with a microplate reader detection wavelength at 450nm, the sample absorbance value and tylosin content is inversely proportional to the sample through the standard curve tylosin content.
3. Kit composed
(1) Pre-coated tylosin coupled antigen removable microtiter plates: 1 block (12 wells × 8 strips)
(2) AFT Standard: 6 bottles (1ml / vial), contents were: 0 ppb, 2 ppb,6 ppb,18ppb,54 ppb,162ppb.
Anti- tylosin antibody conjugate: 1 bottle (6ml)
(4) Color liquid A: 1 bottle (6ml)
(5) Color liquid B: 1 bottle (6ml)
(6) Stop Solution: 1 bottle (6ml), 2M sulfuric acid.
(7) Sample Diluent: 1 bottle (10 ×, 6ml), used for sample dilution.
(8) washing liquid concentrate: 1 bottle (20 ×, 20ml), for the washer
(9) A description
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