1 Use purpose

This kit for Feed, fish, shrimp and meat tissue (such as chicken, beef and pork), eggs, honey, milk, serum and urine in the AHD remaining quantitative detection.

 

2 Experimental principle 

This kit adopts methods in competition ELISA In microplates coated with antigen conjugated AHD, add AHD standard or samples, Free AHD and pre-coated on strips of AHD conjugated antigen compete against AHD antibody conjugates, With TMB chromogenic substrate,the color is changed from blue to yellow after adding stop solution, enzyme standard instrument 450nm wavelengths in testing, absorb light value and in the sample was AHD content is inversely proportional to the standard curve, through calculation samples was AHD concentrations.

 

3 Materials provided with the kit

3.1 Microelisa stripplate: 1 block (12well×8strips).

3.2 AHD standard: six vials of (1ml/ vial), content is respectively: 0 PPB, 0.1 PPB, 0.3 PPB and 0.9 PPB, 2.7 PPB, 8.1 PPB.

3.3 Anti- AHD antibody conjugate: 1 vial (6ml).

3.4 Chromogen Solution A: 1 vial (6ml).

3.5 Chromogen Solution B: 1 vial (6ml).

3.6 Stop Solution: 1 vial (6ml), 2M sulphuric acid.

3.7 sample dilution: 1 vial (10 x, 6ml), used for sample diluted with.

3.8 wash solution: 1 vial (20 x, 20ml), used for washing board.

3.9 Instruction.

 

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