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Drug Names
Generic Name: Horse Growth Differentiation Factor 8 (GDF8) ELISA Kit
Purpose
Our Horse Growth Differentiation Factor 8 (GDF8) ELISA kit is to assay GDF8 levels in Horse serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to GDF8. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for GDF8 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GDF8 and HRP conjugated GDF8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GDF8. You can calculate the concentration of GDF8 in the samples by comparing the OD of the samples to the standard curve.
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