Drug Names

Generic Name: Bovine Herpesvirus 2 (HHV-2) ELISA Kit

Purpose

Our Bovine Herpesvirus 2 (HHV-2) ELISA Kit is for the qualitative determination of HHV-2 in Bovine serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an Antibody specific to HHV-2, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HHV-2 is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain HHV-2 and HRP conjugated HHV-2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of HHV-2 is determined by comparing with the CUTOFF value.

 

 

 

 

 

 

 

 

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