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Mouse Anti-HLA-F antibody
Mouse Anti-HLA-F antibody
CDA12; HLA 5.4; HLA class I histocompatibility antigen, alpha chain F; HLA class I histocompatibility antigen, alpha chain F; HLA class I molecule; HLA F antigen; HLA-CDA12; HLA-F; HLAF; HLAF_HUMAN; Leukocyte antigen F; MHC class I antigen F.
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  • NO.:SLM-51712M
    Clonality:Monoclonal
    Immunogen Species:Mouse
    React Species:Human,
    Applications:WB
    concentration:1mg/ml
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Product Name HLA-F
Chinese Name 组织相容性抗原HLAF单克隆抗体
Alias CDA12; HLA 5.4; HLA class I histocompatibility antigen, alpha chain F; HLA class I histocompatibility antigen, alpha chain F; HLA class I molecule; HLA F antigen; HLA-CDA12; HLA-F; HLAF; HLAF_HUMAN; Leukocyte antigen F; MHC class I antigen F.  
Research Area immunology  
Immunogen Species Mouse
Clonality Monoclonal
Clone NO. H9Y7
React Species Human, 
Applications WB=1:500-1000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 37kDa
Cellular localization The cell membrane 
Form Liquid
Concentration 1mg/ml
immunogen Recombinant human HLA-F. 
Lsotype IgG1,κ
Purification affinity purified by Protein G
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. [provided by RefSeq, Jul 2008]

Function:
Involved in the presentation of foreign antigens to the immune system.

Subunit:
Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).

Subcellular Location:
Membrane..

Similarity:
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

SWISS:
P30511

Gene ID:
3134

Database links:

Entrez Gene: 3134 Human

SwissProt: P30511 Human



Product Picture
Sample:
Lane 1: Raji cell lysates
Primary: Anti-HLA-F (SLM-51712M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 42 kD
Sample:
Lane 1: HepG2 cell lysates
Lane 2: HL-60 cell lysates
Primary: Anti-HLA-F (SLM-51712M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 42 kD

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