Product Name	CCDC46
Chinese Name	卷曲螺旋结构域蛋白46抗体
Alias	CCDC 46; coiled coil domain containing 46; FLJ39610; MGC33887;
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	28kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CCDC46: 751-850/955
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein with filament, myosin tail and ATPase domains. Orthologs of this gene exist in mouse, rat and chimp. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]. Subcellular Location: Cytoplasm, cytoskeleton, centrosome (By similarity). Note=Localizes around spindle poles in some cells. SWISS: Q8N8E3 Gene ID: 201134 Database links: Entrez Gene: 201134 Human Entrez Gene: 76380 Mouse Entrez Gene: 287776 Rat SwissProt: Q8N8E3 Human SwissProt: Q5PR68 Mouse Unigene: 408676 Human Unigene: 344071 Mouse Unigene: 143963 Rat
Product Picture	Sample: U937 Cell (Human) Lysate at 40 ug Primary: Anti- CCDC46 (SL8133R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 26 kD Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CCDC46) Polyclonal Antibody, Unconjugated (SL8133R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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