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Product Name CDCA2 Chinese Name 细胞分裂周期蛋白2抗体 Alias cell division cycle associated 2; Recruits PP1 onto mitotic chromatin at anaphase protein; Repo Man; Repo-Man; CDCA2_HUMAN. Research Area Cell biology Cyclin Cell differentiation Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 113kDa Cellular localization The nucleus The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CDCA2: 361-450/1023 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CDCA2 is a regulator of chromosome structure during mitosis. CDCA2 forms an essential complex with protein phosphatase PP1-gamma subunit 1 (PPP1CC) and is required for the recruitment of PP1 to chromatin. Knockdown of CDCA2 by RNAi causes large-scale cell death by apoptosis, as does overexpression of a dominant-negative mutant of the protein.
Function:
Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.
Subunit:
Interacts with PPP1CC.
Subcellular Location:
Nucleus. Note=Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q69YH5-1; Sequence=Displayed; Name=2; IsoId=Q69YH5-2; Sequence=VSP_025598, VSP_025599; Note=No experimental confirmation available.
Tissue Specificity:
Ubiquitously expressed.
Post-translational modifications:
Phosphorylated by CDK1. May regulate its subcellular location.
SWISS:
Q69YH5
Gene ID:
157313
Database links:Entrez Gene: 157313 Human
SwissProt: Q69YH5 Human
Unigene: 33366 Human
Product Picture Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- CDCA2 (SL7786R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113 kD
Observed band size: 113 kD
Sample:
Siha(Human) Cell Lysate at 30 ug
Primary: Anti- CDCA2 (SL7786R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113 kD
Observed band size: 113 kD
araformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDCA2) Polyclonal Antibody, Unconjugated (SL7786R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CDCA2 Polyclonal Antibody, Unconjugated(SL7786R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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