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Product Name CAPH Chinese Name 染色体相关蛋白H抗体 Alias Barren Drosophila Homolog of 1; Barren homolog protein 1; BRRN; CAP H; CAPH; Chromosome associated protein H; Condensin complex subunit 2; Condensin subunit 2; Condesin; HCAP H; mCAP H; NCAPH; Non SMC condensin I complex subunit H; XCAP H homolog; CND2_HUMAN. Research Area Cell biology Signal transduction Cyclin transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 82kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human BRRN1/CAPH: 601-700/741 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Function:
Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Subunit:
Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: NCAPH/BRRN1, NCAPD2/CAPD2 and NCAPG.
Subcellular Location:
CNucleus. Cytoplasm. Chromosome.
Tissue Specificity:
Widely expressed at low level. Expressed in proliferating cells.
Post-translational modifications:
Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation (By similarity).
Similarity:
Belongs to the CND2 (condensin subunit 2) family.
SWISS:
Q15003
Gene ID:
23397
Database links:Entrez Gene: 23397 Human
Entrez Gene: 215387 Mouse
Omim: 602332 Human
SwissProt: Q15003 Human
SwissProt: Q8C156 Mouse
Unigene: 308045 Human
Unigene: 29786 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CAPH) Polyclonal Antibody, Unconjugated (SL7780R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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