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Product Name IBRDC2 Chinese Name E3Ubiquitin蛋白连接酶144B/Ring finger protein144B抗体 Alias E3 ubiquitin protein ligase RNF144B; RING finger protein 144B; E3 ubiquitin-protein ligase RNF144B; IBR domain containing 2; IBR domain containing protein 2; IBR domain-containing protein 2; IBRDC 2; p53 inducible RING finger protein; p53-inducible RING finger protein; p53RFP; PIR2; R144B_HUMAN; Ring finger 144B; RING finger protein 144B; RNF 144B; RNF144B. Research Area Cell biology Signal transduction Apoptosis Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, Cat, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 34kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human IBRDC2/RING finger protein 144B: 218-303/303 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
Tissue specificity: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
Function:
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
Subunit:
Interacts with UBE2L3, UBE2L6 and LCMT2, as well as with BAX.
Subcellular Location:
Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.
Tissue Specificity:
Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
Post-translational modifications:
Auto-ubiquitinated.
Similarity:
Belongs to the RBR family. RNF144 subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
SWISS:
Q7Z419
Gene ID:
255488
Database links:Entrez Gene: 255488 Human
Entrez Gene: 218215 Mouse
SwissProt: Q7Z419 Human
SwissProt: Q8BKD6 Mouse
Unigene: 148741 Human
Unigene: 287609 Mouse
Unigene: 178363 Rat
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