Product Name	Biglycan
Chinese Name	骨/软骨蛋白多糖1抗体
Alias	BGN; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A.
literatures	Specific References  (4)     |     SL7552R has been referenced in 4 publications. [IF=8.554] Jin Wang. et al. TLR4 aggravates microglial pyroptosis by promoting DDX3X-mediated NLRP3 inflammasome activation via JAK2/STAT1 pathway after spinal cord injury. CLIN TRANSL MED. 2022 Jun;12(6):e894  IP, WB ;  Mouse.   PubMed:35692100 [IF=5.23] Han, Chao, et al. "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." Scientific Reports 6 (2016): 35102.  WB ;  Rabbit.   PubMed:27713567 [IF=2.36] Simões et al. Small leucine-rich proteoglycans (SLRPs) in the endometrium of polycystic ovary syndrome women: a pilot study. (2017) J.Ovarian.Res. 10:54  IHC ;  Human.   PubMed:28789706 [IF=1.72] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014).  IHC-P ;  Rabbit.   PubMed:24987434
Research Area	Cell biology  immunology  Signal transduction  Stem cells  Cytoskeleton  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	37kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Biglycan: 101-200/368
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	May be involved in collagen fiber assembly. Tissue specificity; Found in several connective tissues, especially in articular cartilages. Function: May be involved in collagen fiber assembly (By similarity). Subunit: Homodimer. Forms a ternary complex with MFAP2 and ELN Subcellular Location: Secreted, extracellular space, extracellular. Tissue Specificity: Found in several connective tissues, especially in articular cartilages. Post-translational modifications: The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate (By similarity). Similarity: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats. SWISS: P21810 Gene ID: 633 Database links: Entrez Gene: 633 Human Entrez Gene: 12111 Mouse Entrez Gene: 25181 Rat Omim: 301870 Human SwissProt: P21810 Human SwissProt: P28653 Mouse SwissProt: P47853 Rat Unigene: 821 Human Unigene: 2608 Mouse Unigene: 783 Rat
Product Picture	Sample: Bone (mouse) Lysate at 40 ug Primary: Anti- Biglycan (SL7552R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37kD Observed band size: 52 kD Sample: Colon (Rat) Lysate at 40 ug Embryo (Mouse) Lysate at 40 ug Primary: Anti-Biglycan (SL7552R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 37 kD Observed band size: 52 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(SL7552R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Biglycan) Polyclonal Antibody, Unconjugated (SL7552R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human endothelial cells;4% Paraformaldehyde-fixed; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Biglycan Polyclonal Antibody, Unconjugated(SL7552R) 1:50, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Rhodamine B conjugated (SL0295G-RBITC) used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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Totalgoods,subtotals:￥Checkout