Product Name	PKN1
Chinese Name	蛋白激酶C相关激酶1抗体
Alias	DBK; PAK 1; PAK-1; PAK1; PKC1; PKN ALPHA; PKN; Pkn1; PKN1_HUMAN; PRK1; PRKCL1; Protease activated kinase 1; Protease-activated kinase 1; Protein kinase C like 1; Protein kinase C like PKN; Protein kinase C related kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase N1; Protein kinase PKN alpha; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine threonine kinase N; Serine threonine protein kinase N; Serine-threonine protein kinase N; Serine/threonine protein kinase N1; Serine/threonine-protein kinase N1.
literatures	Specific References  (1)     |     SL7478R has been referenced in 1 publications. [IF=1.785] Xia Wang. et al. Protein kinase N1 promotes proliferation and invasion of liver cancer. Exp Ther Med. 2021 Jun;21(6):1-9  IHC ;  Human.   PubMed:33968181
Research Area	Cell biology  Signal transduction  Kinases and Phosphatases  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Cow, )
Applications	IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	104kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PKN1: 201-300/942
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] Function: PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro. Subcellular Location: Cytoplasm. Nucleus. Endosome. Cell membrane. Cleavage furrow. Midbody. Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Tissue Specificity: Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells. Post-translational modifications: Autophosphorylated; preferably on serine. Phosphorylated during mitosis. Activated by limited proteolysis with trypsin. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats. SWISS: Q16512 Gene ID: 5585 Database links: Entrez Gene: 5585 Human Entrez Gene: 320795 Mouse Entrez Gene: 29355 Rat Omim: 601032 Human SwissProt: Q16512 Human SwissProt: P70268 Mouse SwissProt: Q63433 Rat Unigene: 466044 Human Unigene: 213000 Mouse Unigene: 49880 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PKN1) Polyclonal Antibody, Unconjugated (SL7478R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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