Product Name	DNA polymerase delta
Chinese Name	DNA聚合酶δ 抗体
Alias	DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; DNA polymerase subunit delta 2; DNA polymerase subunit delta p50; DPOD2_HUMAN; POLD 2; POLD2.
Research Area	Cell biology  Chromatin and nuclear signals  Signal transduction  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	51kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from Human DNA polymerase delta: 101-200/469
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). These exonucleases include the family of DNA polymerases (3). DNA pol α, β, ∂, and e are involved in DNA replication and repair (4). DNA pol ∂ and DNA pol e are multisubunit enzymes, with DNA pol ∂ consisting of two subunits p125, which interacts with the sliding DNA clamp protein PCNA, and p50 (5). The nuclear-encoded DNA pol © is the only DNA polymerase required for the replication of the mitochondrial DNA (6). DNA pol Ω is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis (7). DNA pol œ is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks (8). Function: The function of the small subunit is not yet clear. Subcellular Location: Nucleus. Similarity: Belongs to the DNA polymerase delta/II small subunit family. SWISS: P49005 Gene ID: 5425 Database links: Entrez Gene: 281991 Cow Entrez Gene: 5425 Human Entrez Gene: 18972 Mouse Entrez Gene: 289758 Rat Omim: 600815 Human SwissProt: P49004 Cow SwissProt: P49005 Human SwissProt: O35654 Mouse SwissProt: Q6AXY4 Rat Unigene: 306791 Human Unigene: 35788 Mouse Unigene: 22989 Rat
Product Picture	Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-DNA polymerase delta (SL7416R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-DNA polymerase delta (SL7416R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (POLD2) Polyclonal Antibody, Unconjugated (SL7416R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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