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Product Name ARHGEF18 Chinese Name G protein-coupled receptorARHGEF18抗体 Alias p114RhoGEF; 114 kDa Rho specific guanine nucleotide exchange factor; ARHGEF 18; ARHGEF18; KIAA0521; MGC15913; p114 Rho GEF; p114-Rho-GEF; Rho specific guanine nucleotide exchange factor p114; Rho-specific guanine nucleotide exchange factor p114; Rho/Rac guanine nucleotide exchange factor (GEF) 18; Rho/Rac guanine nucleotide exchange factor 18; SA RhoGEF; Septin associated RhoGEF; ARHGI_HUMAN. Research Area Cell biology Signal transduction G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 131kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ARHGEF18/p114RhoGEF: 501-600/1173 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008].
Function:
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RgoGEF cts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. It may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS), but does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production.
Subunit:
Interacts with SEPT9; interaction may inhibit GEF activity. Interacts with Gbetagamma subunits GNB1 and GNG2.
Subcellular Location:
Cytoplasm. Note: Colocalizes with actin stress fibers.
Tissue Specificity:
Expressed in all tissues tested with highest expression in kidney and pancreas. Weakly or not expressed in liver, skeletal muscle and testis.
Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
SWISS:
Q6ZSZ5
Gene ID:
23370
Database links:Entrez Gene: 23370 Human
Entrez Gene: 102098 Mouse
SwissProt: Q6ZSZ5 Human
SwissProt: Q6P9R4 Mouse
Unigene: 465761 Human
Unigene: 736818 Human
Unigene: 170461 Mouse
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