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Rabbit Anti-HSPBAP1 antibody
Rabbit Anti-HSPBAP1 antibody
27 KdA heat shock protein-associated protein 1; HBAP1_HUMAN; hspbap; HSPB1-associated protein 1; HSPBAP1; PASS1; Protein associated with small stress protein 1; protein associating with small stress protein.
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  • NO.:SL6986R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Sheep,)
    Applications:WB ELISA IHC-P IHC-F IF
    concentration:1mg/ml
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Details

Product Name HSPBAP1
Chinese Name 热休克蛋白27相关蛋白1抗体
Alias 27 KdA heat shock protein-associated protein 1; HBAP1_HUMAN; hspbap; HSPB1-associated protein 1; HSPBAP1; PASS1; Protein associated with small stress protein 1; protein associating with small stress protein.  
literatures
Specific References  (1)     |     SL6986R has been referenced in 1 publications.
[IF=2.32] Xu, Shouyu, et al. "Transcriptional profiling of rat skeletal muscle hypertrophy under restriction of blood flow." Gene (2016).  IHC-P ;  Rat.  
Research Area Cell biology  immunology  Signal transduction  Epigenetics  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Sheep, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 54kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human HSPBAP1: 151-250/488 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Heat Shock Protein BAP1 (heat shock 27-kDa-associated protein 1, or HSPBAP1) inhibits the function of heat shock protein 27, which has a neuroprotective effect during experimentally induced epileptic neuropathology. The HSPBAP1 protein, also known as PASS1, contains a jmjC motif, suggesting it is a member of the jumonji family of transcription factors. There is evidence of domain swapping within the jumonji family genes, and the HSPBAP1 gene has been shown to fuse with a novel gene called DIRC3 in hereditary renal cell cancer.

Function:
May play a role in cellular stress response.

Subunit:
Interacts with CRYAB and HSPB1.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Widely expressed.

DISEASE:
Note=A chromosomal aberration involving HSPBAP1 has been found in a family with renal carcinoma. Translocation t(2;3)(q35;q21) with the putative pseudogene DIRC3. Produces an hybrid mRNA encoding a truncated HSPBAP1 lacking the first 36 amino acids.

Similarity:
Contains 1 JmjC domain.

SWISS:
Q96EW2

Gene ID:
79663

Database links:

Entrez Gene: 79663 Human

Omim: 608263 Human

SwissProt: Q96EW2 Human

Unigene: 29169 Human



Product Picture
Sample:
293T Cell(human) Lysate at 40 ug
Lymph node (mouse) Lysate at 40 ug
HepG2 Cell(human) Lysate at 40 ug
Testis (mouse) Lysate at 40 ug
Primary: Anti- HSPBAP1(SL6986R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated (SL6986R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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