Product Name	FOXN1
Chinese Name	叉头蛋白N1抗体
Alias	FKHL20; Forkhead box N1; Forkhead box protein N1; FOXN 1; FOXN1; FOXN1_HUMAN; RONU; Rowett nude; Transcription factor winged-helix nude; WHN; Winged helix nude; Winged-helix transcription factor nude.
literatures	Specific References  (7)     |     SL6970R has been referenced in 7 publications. [IF=47.99] Zeleniak, Ann. et al. De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids. NAT METHODS. 2022 Sep;:1-14  IF ;  Human.   PubMed:36064772 [IF=11.99] Volpi, Stefano, et al. "EXTL3 mutations cause skeletal dysplasia, immune deficiency, and developmental delay." Journal of Experimental Medicine (2017): jem-20161525.  IF(ICC) ;  Human.   PubMed:28148688 [IF=6.142] Kolb AD et al. Osteoblasts are “educated” by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment.Breast Cancer Res. 2019 Feb 27;21(1):31.  WB/IHF ;  Human &Mouse.   PubMed:30813947 [IF=4.932] Shu-ping Yang. et al. Metformin ameliorates thymus degeneration of mice by regulating mitochondrial function. INT IMMUNOPHARMACOL. Int Immunopharmacol. 2022 Jul;108:108744  IHC ;  Mouse.   PubMed:35395467 [IF=3.297] Yuyan Wang. et al. ET-1 regulates the human umbilical vein endothelial cell cycle by adjusting the ERβ/FOXN1 signaling pathway. Ann Transl Med. 2020 Nov; 8(22): 1499  WB ;  Human.   PubMed:33313244 [IF=2.798] Guo L et al. Gallic acid attenuates thymic involution in the D-galactose induced accelerated aging mice. Immunobiology. 2019.  IHC-P ;  Mouse.   PubMed:doi:10.1016/j.imbio.2019.11.005 [IF=1.662] Ting‐ting Wei. et al. Dosage effects of resveratrol on thymus involution in D‐galactose‐treated mice. 2021 Mar 28  IHC ;  Mouse.   PubMed:33778958
Research Area	Cell biology  immunology  Chromatin and nuclear signals  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	69kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FOXN1: 321-420/648
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008]. Function: Transcriptional regulator involved in development. Subcellular Location: Nucleus. Tissue Specificity: Expressed in thymus. Similarity: Contains 1 fork-head DNA-binding domain. SWISS: O15353 Gene ID: 8456 Database links: Entrez Gene: 8456 Human Omim: 600838 Human SwissProt: O15353 Human Unigene: 663679 Human
Product Picture	Sample: Thymus (Rat) Lysate at 40 ug Primary: Anti-FOXN1 (SL6970R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 69 kD Observed band size: 69 kD Paraformaldehyde-fixed, paraffin embedded (Mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FOXN1) Polyclonal Antibody, Unconjugated (SL6970R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FOXN1 Polyclonal Antibody, Unconjugated(SL6970R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Hela. Primary Antibody (green line): Rabbit Anti-FOXN1 antibody (SL6970R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (Black line): Hela (Black). Primary Antibody (green line): Rabbit Anti-FOXN1 antibody (SL6970R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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