Product Name	SEMA4D
Chinese Name	Transmembrane proteinSEMA4D抗体
Alias	Semaphorin-4D; Semaphorin4D; A8 antibody BB 18; BB18; CD 100; CD100; CD100 antigen; Coll 4; COLL4; Collapsin 4; Collapsin4; GR3; Human semaphorin CD100 mRNA complete cds; Leukocyte activation antigen CD100; M sema G; MSEMA; SEM4D_HUMAN; Sema 4d; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D ; Sema H; SEMA J; Semacl 2; Semacl2; SemaH; SEMAJ; Semaphorin 4D; Semaphorin 4D precursor; Semaphorin C like 2; Semaphorin H; Semaphorin J; SemaphorinJ; Semcl 2; Semcl2.
Research Area	Cell biology  immunology  Neurobiology  Cell type markers
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	60, 126kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SEMA4D: 75-170/862 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	May play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro. Subunit: Homodimer. Interacts with PLXNB2 (By similarity). Binds PLXNB1. Subcellular Location: Membrane. Tissue Specificity: Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. Similarity: Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain. SWISS: Q92854 Gene ID: 10507 Database links: Entrez Gene: 10507 Human Omim: 601866 Human SwissProt: Q92854 Human Unigene: 494406 Human
Product Picture	Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-SEMA4D (SL6965R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60/126 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SEMA4D) Polyclonal Antibody, Unconjugated (SL6965R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: Raji(blue). Primary Antibody:Rabbit Anti-SEMA4D antibody(SL6965R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (SL6965R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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