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Product Name Cyclin M2 Chinese Name 周期素M2抗体 Alias ACDP2; Ancient conserved domain containing protein 2; Ancient conserved domain protein 2; Ancient conserved domain-containing protein 2; CNNM 2; CNNM2; CNNM2_HUMAN; Cyclin M2; Cyclin-M2; Metal transporter CNNM2; OTTHUMP00000020387; OTTHUMP00000020388. literatures Specific References (1) | SL6912R has been referenced in 1 publications.[IF=4.196] Takashina Y et al. Sodium Citrate Increases Expression and Flux of Mg Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells.(2018) Nutrients. Sep 20 ;10(10) WB ; Rat.Research Area Cell biology immunology Signal transduction Cyclin Channel protein The cell membrane受体 Cell differentiation Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 96kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Cyclin M2/CNNM2: 51-150/875 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cyclin M2, also known as CNNM2 or ACDP2 (ancient conserved domain-containing protein 2), is an 875 amino acid multi-pass membrane protein that contains two CBS domains and belongs to the ACDP family. Expressed in a variety of tissues with highest expression in placenta, brain and kidney, cyclin M2 functions as a divalent metal cation transporter that mediates the transport of several different metal cations, including Mg2+, Co2+ and Fe2+. Cyclin M2 exists as multiple alternatively spliced isoforms and, contrary to its name, exhibits no cyclin-like function in vivo. The gene encoding cyclin M2 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.
Function:
Divalent metal cation transporter. Mediates transport of divalent metal cations in an order of Mg(2+) > Co(2+) > Mn(2+) > Sr(2+) > Ba(2+) > Cu(2+) > Fe(2+
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
Widely expressed. Expressed at higher level in brain, kidney and placenta, while it is weakly expressed in skeletal muscle. In the kidney, it is expressed in the distal convoluted tubule and the thick ascending limb of Henle loop.
DISEASE:
Defects in CNNM2 are the cause of hypomagnesemia type 6 (HOMG6) [MIM:613882]. A renal disease characterized by severely lowered serum magnesium levels in the absence of other electrolyte disturbances. Affected individuals show an inappropriately normal urinary magnesium excretion, demonstrating a defect in tubular reabsorption. Age of clinical onset is highly variable and some affected individuals are asymptomatic.
Similarity:
Belongs to the ACDP family.
Contains 2 CBS domains.
Contains 1 DUF21 domain.
SWISS:
Q9H8M5
Gene ID:
54805
Database links:Entrez Gene: 54805 Human
Entrez Gene: 94219 Mouse
Omim: 607803 Human
SwissProt: Q9H8M5 Human
SwissProt: Q3TWN3 Mouse
Unigene: 643509 Human
Unigene: 657970 Human
Unigene: 306903 Mouse
Unigene: 205139 Rat
Product Picture Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin M2 (SL6912R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 96 kD
Observed band size: 96 kD
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Cyclin M2 antibody (SL6912R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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