Product Name	BOD1
Chinese Name	双向染色体细胞分裂蛋白1抗体
Alias	Biorientation defective 1; Biorientation defective protein 1; biorientation of chromosomes in cell division 1; Biorientation of chromosomes in cell division protein 1; BOD1; BOD1_HUMAN; FAM44B; family with sequence similarity 44, member B; Protein FAM44B.
Research Area	Cell biology  Cyclin  Cell differentiation  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	20kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human BOD1: 51-150/185
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Required for proper chromosome biorientation through the detection or correction of syntelic attachments in mitotic spindles. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Note=Localizes at the centrosomes throughout the cell cycle, only dissociating during cytokinesis. Localizes at the kinetochore from prometaphase until anaphase. Similarity: Belongs to the BOD1 family. SWISS: Q96IK1 Gene ID: 91272
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BOD1 Polyclonal Antibody, Unconjugated(SL6900R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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