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Product Name TPST1 Chinese Name 酪蛋白硫酸转移酶1抗体 Alias Protein tyrosine sulfotransferase 1; Protein-tyrosine sulfotransferase 1; TPST-1; TPST1_HUMAN; Tyrosylprotein sulfotransferase 1. Research Area Cardiovascular Cell biology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 41kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TPST1: 165-260/370 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Function:
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
Subcellular Location:
Golgi apparatus membrane; Single-pass type II membrane protein.
Similarity:
Belongs to the protein sulfotransferase family.
SWISS:
O60507
Gene ID:
8460
Product Picture Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-TPST1 (SL6850R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 51 kD
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TPST1 Polyclonal Antibody, Unconjugated(SL6850R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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