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Product Name MAGEB1 Chinese Name 黑色素瘤相关抗原B1抗体 Alias Cancer/testis antigen 3.1; CT3.1; DAM10; DSS AHC critical interval MAGE superfamily 10; MAGE B1 antigen; MAGE Xp; MAGEL1; MAGEXP antigen; melanoma antigen family B, 1; Melanoma associated antigen B1. Research Area Tumour Cell type markers TumourCell biologyMaker Immunogen Species Rabbit Clonality Polyclonal React Species Human, Dog, (predicted: Mouse, Rat, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 39kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MAGEB1: 1-100/347 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail MAGEB1 is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region, and expressed in testis and in a significant fraction of tumors of various histological types. This gene and other MAGEB members are clustered on chromosome Xp22-p21.
Tissue Specificity:
Expressed only in testis.
Similarity:
Contains 1 MAGE domain.
SWISS:
Q96M61
Gene ID:
286514
Database links:Entrez Gene: 286514 Human
SwissProt: Q96M61 Human
Unigene: 350683 Human
Product Picture Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-MAGEB1 (SL6821R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-MAGEB1 (SL6821R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Tissue/cell: Dog testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-MAGEB1 Polyclonal Antibody, Unconjugated(SL6821R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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