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Product Name APPD Chinese Name 凋亡诱导蛋白D抗体 Alias PKHF1_HUMAN; Apoptosis inducing protein D; LAPF; Lysosome associated apoptosis inducing protein containing PH and FYVE domains; MGC4090; PH and FYVE domain containing protein 1; PH domain containing family F member 1; Phafin 1; Pleckstrin homology domain containing family F (with FYVE domain) member 1; Pleckstrin homology domain containing family F member 1; Pleckstrin homology domain containing family F with FYVE domain member 1; PLEKHF1; ZFYVE15; Zinc finger FYVE domain containing protein 15. Research Area Cell biology Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Rabbit, Sheep, ) Applications IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 31kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human APPD/LAPF: 75-124/279 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail APPD is a lysosome-associated apoptosis-inducing protein containing the pleckstrin homology (PH) and FYVE domains (LAPF). It is a representative of a novel family of PH and FYVE domain-containing proteins and induces caspase-independent apoptosis via the lysosomal-mitochondrial pathway. Cellular localization: Nucleus. Cytoplasm, perinuclear region. Lysosome. Note=Translocates to lysosome during apoptosis.
Function:
May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
Subcellular Location:
Nucleus. Cytoplasm, perinuclear region. Lysosome. Note=Translocates to lysosome during apoptosis.
Tissue Specificity:
Highly expressed in heart and skeletal muscle. Weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung.
Similarity:
Contains 1 FYVE-type zinc finger.
Contains 1 PH domain.
SWISS:
Q96S99
Gene ID:
79156
Database links:Entrez Gene: 79156 Human
Entrez Gene: 72287 Mouse
SwissProt: Q96S99 Human
SwissProt: Q3TB82 Mouse
Unigene: 466383 Human
Unigene: 58389 Human
Unigene: 333798 Mouse
Unigene: 9380 Rat
Product Picture Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APPD Polyclonal Antibody, Unconjugated(SL6793R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APPD Polyclonal Antibody, Unconjugated(SL6793R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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