Rabbit Anti-SLC26A4 antibody
PDS; deafness, autosomal recessive 4; DFNB4; EVA; NSRD4; Pendred syndrome; Pendred syndrome homolog; Pendrin; S26A4_HUMAN; SLC26A4; Sodium independent chloride/iodide transporter; Sodium-independent chloride/iodide transporter; Solute carrier family 26 me
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Product Name SLC26A4 Chinese Name 钠碘单独TransporterSLC26A4抗体 Alias PDS; deafness, autosomal recessive 4; DFNB4; EVA; NSRD4; Pendred syndrome; Pendred syndrome homolog; Pendrin; S26A4_HUMAN; SLC26A4; Sodium independent chloride/iodide transporter; Sodium-independent chloride/iodide transporter; Solute carrier family 26 member 4. literatures Research Area Cell biology Neurobiology Signal transduction transcriptional regulatory factor Channel protein Cell Surface Molecule Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 93kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Solute carrier family 26 member 4: 301-400/780 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene belongs to the solute carrier 26 family, whose members encode anion transporter proteins. This particular family member encodes a protein involved in transporting chloride, oxalate, sulfate and bicarbonate. Several alternatively spliced transcript variants of this gene, encoding distinct isoforms, have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008].
Function:
Sodium-independent transporter of chloride and iodide.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.
DISEASE:
Defects in SLC26A4 are the cause of deafness autosomal recessive type 4 (DFNB4) [MIM:600791]; also known as vestibular aqueduct syndrome (EVA). DFNB4 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNB4 is associated with an enlarged vestibular aqueduct.
Similarity:
Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. Contains 1 STAS domain.
SWISS:
O43511
Gene ID:
5172
Database links:
UniProtKB/Swiss-Prot: O43511.1
Product Picture 
Sample:
Lane 1: Human TT cell lysates
Lane 2: Human U251 cell lysates
Primary: Anti-SLC26A4 (SL6787R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 93 kDa
Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-SLC26A4 antibody (SL6787R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-SLC26A4 antibody (SL6787R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Black line : Positive blank control (U87MG); Negative blank control (Molt4）
Green line : Primary Antibody (Rabbit Anti-SLC26A4 antibody (SL6787R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488)
U87MG（Positive）and Molt4（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SLC26A4 Antibody(SL6787R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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