Product Name	NALP3/CIAS1
Chinese Name	Apoptosis诱导蛋白NALP3抗体
Alias	LRR and PYD domains-containing protein 3; AGTAVPRL; AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLRP3; PYPAF 1; PYPAF1 antibody PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1.
literatures	Specific References  (20)     |     SL6655R has been referenced in 20 publications. [IF=10.761] Zineng Yan. et al. Engineering exosomes by three-dimensional porous scaffold culture of human umbilical cord mesenchymal stem cells promotes osteochondral repair. MATER TODAY BIO. 2023 Jan;:100549  IF ;  Mouse.   PubMed:36756208 [IF=8.131] Runxiao Zheng. et al. Particulate matter aggravates Alzheimer's disease by activating the NLRP3 inflammasome to release ASC specks. Environ Sci-Nano. 2021 Jun;:  WB ;  Mouse.   PubMed:10.1039/D1EN00361E [IF=6.922] Wei Zhou. et al. Computational Systems Pharmacology, Molecular Docking and Experiments Reveal the Protective Mechanism of Li-Da-Qian Mixture in the Treatment of Glomerulonephritis. J Inflamm Res. 2021 Dec;14:6939-6958  WB ;  Mouse.   PubMed:34949932 [IF=6.817] Shuaitao Yang. et al. Blockage of transient receptor potential vanilloid 4 prevents postoperative atrial fibrillation by inhibiting NLRP3-inflammasome in sterile pericarditis mice. CELL CALCIUM. Cell Calcium. 2022 Apr;:102590  WB ;  Mouse.   PubMed:35439615 [IF=6.304] Chen Y et al. Dendritic cells-derived interferon-λ1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414.  WB ;  Mouse.   PubMed:32488049 [IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078  WB ;  Mouse.   PubMed:35489469 [IF=5.639] Song Xue. et al. Receptor-Interacting Protein Kinase 3 Inhibition Relieves Mechanical Allodynia and Suppresses NLRP3 Inflammasome and NF-κB in a Rat Model of Spinal Cord Injury. FRONT MOL NEUROSCI. 2022; 15: 861312  IF ;  Rat.   PubMed:35514432 [IF=4.858] Kun Hao. et al. Targeting BRD4 prevents acute gouty arthritis by regulating pyroptosis. Int J Biol Sci. 2020; 16(16): 3163–3173  WB ;  Rat, Human.   PubMed:33162822 [IF=4.545] Zhao J et al. Cepharanthine attenuates cerebral ischemia/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12/15-LOX signaling. Biomed Pharmacother. 2020 Jul;127:110151.  IF ;  Mouse.   PubMed:32559840 [IF=4.511] Xi-min Hu. et al. Pyroptosis-related gene signature elicits immune response in rosacea. EXP DERMATOL. 2023 Apr;:  IHC ;  Human.   PubMed:37086043 [IF=4.321] Shu-Min Yeet al. Silencing of Gasdermin D by siRNA-Loaded PEI-Chol Lipopolymers Potently Relieves Acute Gouty Arthritis through Inhibiting Pyroptosis. Mol Pharm . 2020 Jul 6.  WB ;  mouse.   PubMed:32579365 [IF=4.268] Wenwen Liu. et al. Effects of andrographolide on renal tubulointersticial injury and fibrosis. Evidence of its mechanism of action. Phytomedicine. 2021 Jul;:153650  WB ;  Human.   PubMed:34332282 [IF=3.264] Zhang Haoyun. et al. Spatiotemporal evolution of pyroptosis and canonical inflammasome pathway in hSOD1G93A ALS mouse model. BMC NEUROSCI. 2022 Dec;23(1):1-14  IF, WB ;  Mouse.   PubMed:35945502 [IF=2.984] Yitong Pan. et al. N-acetyl-L-tryptophan attenuates hepatic ischemia-reperfusion injury via regulating TLR4/NLRP3 signaling pathway in rats. Peerj. 2021 Aug;9:e11909  WB,IF ;  Rat.   PubMed:34434653 [IF=2.388] Xiaohong Xu. et al. Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway. Bmc Nephrol. 2021 Dec;22(1):1-17  IF ;  Mouse.   PubMed:34107901 [IF=2.264] Yu Zhang. et al. Blocking CXC Motif Chemokine Ligand 2 Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Apoptosis and NLRP3 Inflammasome Activation. BIOL PHARM BULL. 2023 May;46(5):672-683  ICC ;  Rat.   PubMed:37121693 [IF=2.1] Xianzhen Zhang. et al. Simiaosan alleviates the symptoms of gouty arthritis via the NALP3/IL‑1β pathway. Mol Med Rep. 2021 Mar;23(3):1-1  WB,IHC ;  Rat.   PubMed:33655332 [IF=1.652] Wang Y et al. Pre-administration of luteoline attenuates neonatal sevoflurane-induced neurotoxicity in mice. Acta Histochem. 2019 May;121(4):500-507.  WB ;  Mouse.   PubMed:31006528 [IF=0.439] Chen JW et al. Zhihuang Tongfeng decoction ameliorates gouty arthritis via inhibition of NLRP3 inflammasome in rats. Tropical Journal of Pharmaceutical Research December 2019; 18 (12): 2577-2582.  WB ;  Rat.   PubMed:doi:10.4314/tjpr.v18i12.17 [IF=]   WB ;  mouse.   PubMed:10.1002/ctm2.240
Research Area	Cardiovascular  Cell biology  immunology  Signal transduction  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	114kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Cryopyrin: 551-630/1036
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. Similarity: Belongs to the NLRP family. Contains 1 DAPIN domain. Contains 9 LRR (leucine-rich) repeats. Contains 1 NACHT domain. SWISS: Q96P20 Gene ID: 114548 Database links: Entrez Gene: 114548 Human Entrez Gene: 216799 Mouse Entrez Gene: 287362 Rat Omim: 606416 Human SwissProt: Q96P20 Human SwissProt: Q8R4B8 Mouse Unigene: 159483 Human Unigene: 54174 Mouse
Product Picture	Sample: Lung (Rat) Lysate at 40 ug Colon carcinoma (Human) Lysate at 40 ug Primary: Anti-NALP3/CIAS1 (SL6655R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 114 kD Observed band size: 114 kD Tissue/cell: Rat tracheal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cryopyrin Polyclonal Antibody, Unconjugated(SL3301R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control（black line）:Raw264.7. Primary Antibody (green line): Rabbit Anti-NALP3/CIAS1 antibody (SL6655R) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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